qubit protein broad range assay kit (Thermo Fisher)

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Thermo Fisher qubit protein broad range assay kit
Qubit Protein Broad Range Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+protein+broad+range+assay+kit/qubit+dsdna+hs+assay+kit/bio_rxiv__2025__07__12__664526-216-5-11
Average 90 stars, based on 1 article reviews

qubit protein broad range assay kit - by Bioz Stars, 2026-09

90/100 stars

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Protein Concentration:

Article Title: Integrative characterization of MYC RNA-binding function
Article Snippet: When necessary, Amicon Ultra-4 Centrifugal Filters (Millipore, UFC801008) were used to concentrate the volumes of liquid. .. Final protein concentration was quantified using Qubit Protein Broad Range Assay Kit (ThermoFisher, A50668) with Qubit 4 Fluorometer and then stored in aliquots at −80°C avoiding repeated thawing. ..

Article Title: Phosphorylation by the PknB serine/threonine kinase stimulates dimer formation by WalR
Article Snippet: .. Protein concentration was measured using Qubit Protein Broad Range Assay Kit (Thermo Fisher), concentrated if needed using Amicon Ultra Centrifugal Filters 10 kDa MWCO (Millipore), aliquoted, snap frozen in liquid nitrogen, and stored at -80°C until required. ..

Article Title: Targeting Semaphorin 7a signaling in preclinical models of estrogen receptor-positive breast cancer
Article Snippet: .. Protein concentration of lysates was quantified using Qubit Protein Broad Range Assay Kit (ThermoFisher; Cat# A50668). .. Per sample, 1.0 mg of lysate was pre-incubated with 30 uL IP antibody: mouse IgG or SEMA7A (Santa Cruz; Sc-374432) and rocked overnight at 4°C.

other:

Article Title: Integrative characterization of MYC RNA-binding function.
Article Snippet: Electrophoretic mobility shift assay (EMSA) For RNA EMSA, binding assays were performed as previously described with slight modification.75 All RNA oligos were synthesized with HLPC-graded purification from Sigma (Table S8) and labeled with the 5′ EndTag DNA/RNA Labeling Kit (Vector Laboratories, MB9001) and a fluorescence dye, DyLight 800 Maleimide (ThermoFisher, 46621).

DC Protein Assay:

Article Title: Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis
Article Snippet: .. Whole cell lysates were prepared in RIPA buffer (Cell Signaling Technology) as previously described and protein concentrations were measured using the DC Protein Assay (Bio-Rad) or the Qubit Protein Broad Range Assay kit (Thermo Fisher). .. For AML12 cell lysates, ~5×10 6 cells cultured in a 10 cm dish were harvested by scraping in 1X RIPA buffer with 1X HALT Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) and 1 mM PMSF and centrifuged (5 min at 13,000 rpm).

Article Title: Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis.
Article Snippet: .. Whole cell lysates were prepared in RIPA buffer (Cell Signaling Technology) as previously described81 and protein concentrations weremeasured using the DC Protein Assay (Bio-Rad) or the Qubit Protein Broad Range Assay kit (Thermo Fisher). .. For AML12 cell lysates, 5x106 cells cultured in a 10 cm dish were harvested by scraping in 1X RIPA buffer with 1X HALT Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) and 1 mM PMSF and centrifuged (5min at 13,000 rpm).

Concentration Assay:

Article Title: A virally-encoded tRNA neutralizes the PARIS antiviral defence system.
Article Snippet: Nathaniel Burman, Svetlana Belukhina, Florence Depardieu, Royce A. Wilkinson, Mikhail Skutel, Andrew Santiago-Frangos, Ava B. Graham, Alexei Livenskyi, Anna Chechenina, Natalia Morozova, Trevor Z ah l , W il li am S. Henriques, Murat Buyukyoruk, Christophe Rouillon, Baptiste Saudemont, Lena Shyrokova, Tatsuaki Kurata, Vasili H au ry li uk , K on stantin Severinov, Justine Groseille, Agnès Thierry, Romain Koszul, Florian Tesson, Aude Bernheim, David Bikard, Blake Wiedenheft & Artem Isaev

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